Takara primescripttm rt master mix
WebSep 16, 2015 · The reverse transcription was performed using the Takara PrimeScriptTM RT Master Mix (Perfect Real Time) according to the manufacturer’s instructions. Quantitative real-time PCR was performed as previously described . The relative mRNA quantity was calculated using the ΔΔCt method . Gene expression was normalized to … WebFeb 1, 2024 · Complementary DNA (cDNA) was synthesized using the TAKARA PrimeScriptTM RT reagent Kit (TAKARA, RR047A). qRT-PCR was performed using SYBR® Select Master Mix (Applied Biosystems). The primers sequences were designed as follows: TRIM24, forward 5′-CAGCCACAAATGCCTAAGCAG-3′, reverse 5′ …
Takara primescripttm rt master mix
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WebTotal RNA was extracted from colorectal cancer and tissues using TRIzol (TaKaRa, Shiga, Japan) according to the manufacturer’s instructions. TOB1 and GAPDH mRNA were reversely transcribed into cDNA with a PrimeScriptTM RT Master Mix reagent kit (TaKaRa). miR-32-5p and U6 were reversely transcribed into cDNA by the … Web本发明适用于蜜蜂气味受体研究技术领域，提供了中华蜜蜂气味受体AcerOr2对精液品质的影响分析的实验方法，首先通过进行荧光定量检测目的基因的表达，然后进行Western blot检测目的基因蛋白的表达，接着使用CASA计算机辅助分析仪评估精子常规指标，最后通过流式细胞仪检测精子DNA完整性，从而 ...
WebMar 1, 2024 · 本制品是2 Step Real Time RT-PCR用的理想反转录反应试剂。. 5×PrimeScript RT Master Mix中含有定量RT-PCR的反转录反应所需的所有试剂 (PrimeScript RTase … WebMaxima RT is designed for enhanced consistency and efficiency in cDNA synthesis for both RT-PCR and RT-qPCR applications in a variety of formats (e.g., stand-alone enzymes, RT kits and master mixes). Maxima RT kits and master mixes are also available with an integrated gDNA removal step for efficient and simplified workflow.
Web1.1药物与试剂MUTZ-1细胞购自北京协和细胞库；重组人IL-23购自美国R&D公司；人淋巴细胞分离液购自武汉博士德生物工程有限公司；RPMI1640培养基、胎牛血清(FBS)购自美国Gibco公司；IFN-γ、TNF-α、TGF-β、颗粒酶A、颗粒酶B、穿孔素一抗购自美国Invitrogen公司；PrimeScriptTM ... WebApr 15, 2024 · Then, 500 ng of total RNA was reverse-transcribed into single-strand cDNA with PrimeScript RT Master Mix (TaKaRa, Japan). Real-time PCR was performed using LightCycler ® 480 SYBR ® Green Ⅰ Master Mix (Roche, catalog no. 04707516001) on a CFX Connect™ Real-Time system, and the results were analyzed with CFX Maestro™ …
WebQuickCleanTM Enzyme Removal Resin (TaKaRa Code 631770) SUPREC®-01 (TaKaRa Code 9040) TaKaRa RECOCHIP (TaKaRa Code 9039) ＜PCR 효소> PrimeSTAR® HS DNA Polymerase (TaKaRa Code R010A) ＜Insert 확인용＞ EmeraldAmp® PCR Master Mix (TaKaRa Code RR300A) SapphireAmp® Fast PCR Master Mix (TaKaRa Code …
WebOct 12, 2024 · Reverse transcription of total RNA was performed using Takara PrimeScriptTM RT Master Mix. RT-qPCR for the specific gene was performed using Takara TB-Green Premix Ex Taq in the LightCycler RT-qPCR system (LC-480 II, Roche, Switzerland). The primers used in this study can be found in Table S2. bear lake parking rmnpbear lake raspberry days 2023WebJun 18, 2024 · Briefly, the total RNA was extracted by RNAiso Plus (Takara, Tokyo, Japan) according to the manufacturer’s instructions, which was used as the template for cDNA synthesis by using PrimeScriptTM RT Master Mix (Perfects Real Time; Takara). diamond\u0027s jfWeb丁香通为您找到12条trizol试剂takara信息，包括trizol试剂takara报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购trizol试剂takara,上丁香通。 ... TaKaRa RR036A PrimeScript RT Master Mix ... diamond\u0027s izWebMar 1, 2024 · Takara. RR037B (A × 4) PrimeScript ™ RT reagent Kit (Perfect Real Time) 200 Rxns × 4. ￥7,456. ￥4,474. *红字为促销价格，促销时间: 2024年3月1日-2024年4月30日. 收藏产品 加入购物车. bear lake raspberry jamWebJun 22, 2024 · SYBR Green Realtime PCR Master Mix kit was used for quantitative analysis, and each sample was replicated three times. Reaction system 20 μL: 2 μL cDNA, upstream and downstream primers (10 μmol/L) 2 μL, 10 μL SYBR, 6 μL ddH 2 O. Reaction procedure: pre-denaturation at 95 °C for 30 s, denaturation at 95 °C for 10 s, annealing … bear lake park idahohttp://zjzy.yzu.edu.cn/download.php?id=bm5ELTZsZ2dRa2pkUlV1WT0zdnlUakR0RTJ5QnZudW1y bear lake polar plunge